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Synthesis of quantum dots for in-vivo imaging
Ferdusová, Helena ; Majzlíková, Petra (referee) ; Pekárková, Jana (advisor)
The aim of this work was to synthesise water-soluble QDs using different precursors and stabilizers and to determine the toxicity of the synthesized QDs by in vivo imaging. Experiments were performed on water-soluble QDs (MPA-CdTe, MPA-CdTe/ZnS, MSA-CdTe, MSA-CdTe/ZnS, GSH-CdTe, GSH-CdTe/ZnS, TGA-CdTe, TGA-CdTe/ZnS, GSH-ZnSe and GSH-ZnSe/ZnS ) and toxicity was measured. Synthesized QDs were characterized by high intensity (fluorescence spectroscopy), FWHM and zeta potential (ZS Zetasizer) were selected due to their suitability for this task. The toxicity of QDs was determined by the MTT assay on the cell line HEK 293. The experiments show that a core/shell structure is less toxic than a core structure. The results indicate that the toxicity of our synthesized QDs is the lowest for MPA-CdTe (core structure) and MPA-CdTe/ZnS (core/shell structure).
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Synthesis of colloidal gold nanorods for biomedical applications
Valkovičová, Jiřina ; Bernard, Vladan (referee) ; Drbohlavová, Jana (advisor)
Diplomová práce se zabývá výrobou a využitím zlatých koloidních nanotyčinek v biomedicínských aplikacích. Konkrétně se zaměřuje na tři základní funkce nanočástic v analýze - transportní, separační a především zobrazovací. V části o využití nanočástic pro zobrazování je hlavní zájem věnován zejména povrchové plazmonové rezonanci zlatých nantyčinek. Dále je práce zaměřena na využití nanotyčinek pro termální terapii. Následující část je věnována vhodným modifikacím povrchu, hlavně za účelem zmírnění toxicity částic. Závěrem teoretické části jsou způsoby přípravy zlatých koloidních nanotyčinek a techniky jejich následné analýzy. V navazující experimetální části jsou uvedeny postupy, podmínky a výsledky provedených pokusů.
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Interaction of animal cells with organic semiconductors
Schildová, Veronika ; Thalerová, Sandra (referee) ; Víteček,, Jan (advisor)
This bachelor´s thesis was focused on study of biocompatibility of organic semiconductors. In introduction, organic semiconductors and their properties, benefits and features of selected representatives were described. In the following part biocompatibility definition and approaches for its assays including tests of cytotoxicity, material stability were presented. The last part was focused on overview of cell cultures, appropriate cultivation conditions and compounds of media for in vitro testing of biocompatibility. Experimental part showed the result of biocompatibility testing of materials. For this purpose murine 3T3 fibroblasts were used.
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Interaction of animal cells with organic semiconductors
Schildová, Veronika ; Thalerová, Sandra (referee) ; Víteček,, Jan (advisor)
This bachelor´s thesis was focused on study of biocompatibility of organic semiconductors. In introduction, organic semiconductors and their properties, benefits and features of selected representatives were described. In the following part biocompatibility definition and approaches for its assays including tests of cytotoxicity, material stability were presented. The last part was focused on overview of cell cultures, appropriate cultivation conditions and compounds of media for in vitro testing of biocompatibility. Experimental part showed the result of biocompatibility testing of materials. For this purpose murine 3T3 fibroblasts were used.
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Antivirotické účinky stilbenoidů proti klíšťaty přenášeným patogenům in vivo
MAŠKOVÁ, Hana
This study was focused on antiviral effects of stilbenoids against a virus transmitted by ticks. The cell viability of selected cell line cultures in the presence of various concentrations of stilbenoids was determined using the MTT assay. Similarly, the mixed effect of other known antiviral substances and stilbenoids was studied using the MTT assay. Both the prophylactic effects of stilbenoids on the infected culture cell lines and the effect on viral replication were examined. The viral titres from samples were determined using plaque assay. Some of the experiments were performed also in vivo using laboratory mice.
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Synthesis of quantum dots for in-vivo imaging
Ferdusová, Helena ; Majzlíková, Petra (referee) ; Pekárková, Jana (advisor)
The aim of this work was to synthesise water-soluble QDs using different precursors and stabilizers and to determine the toxicity of the synthesized QDs by in vivo imaging. Experiments were performed on water-soluble QDs (MPA-CdTe, MPA-CdTe/ZnS, MSA-CdTe, MSA-CdTe/ZnS, GSH-CdTe, GSH-CdTe/ZnS, TGA-CdTe, TGA-CdTe/ZnS, GSH-ZnSe and GSH-ZnSe/ZnS ) and toxicity was measured. Synthesized QDs were characterized by high intensity (fluorescence spectroscopy), FWHM and zeta potential (ZS Zetasizer) were selected due to their suitability for this task. The toxicity of QDs was determined by the MTT assay on the cell line HEK 293. The experiments show that a core/shell structure is less toxic than a core structure. The results indicate that the toxicity of our synthesized QDs is the lowest for MPA-CdTe (core structure) and MPA-CdTe/ZnS (core/shell structure).
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Synthesis of colloidal gold nanorods for biomedical applications
Valkovičová, Jiřina ; Bernard, Vladan (referee) ; Drbohlavová, Jana (advisor)
Diplomová práce se zabývá výrobou a využitím zlatých koloidních nanotyčinek v biomedicínských aplikacích. Konkrétně se zaměřuje na tři základní funkce nanočástic v analýze - transportní, separační a především zobrazovací. V části o využití nanočástic pro zobrazování je hlavní zájem věnován zejména povrchové plazmonové rezonanci zlatých nantyčinek. Dále je práce zaměřena na využití nanotyčinek pro termální terapii. Následující část je věnována vhodným modifikacím povrchu, hlavně za účelem zmírnění toxicity částic. Závěrem teoretické části jsou způsoby přípravy zlatých koloidních nanotyčinek a techniky jejich následné analýzy. V navazující experimetální části jsou uvedeny postupy, podmínky a výsledky provedených pokusů.
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Synthesis of quantum dots for in-vivo imaging
Ferdusová, Helena ; Majzlíková, Petra (referee) ; Pekárková, Jana (advisor)
The aim of this work was to synthesise water-soluble QDs using different precursors and stabilizers and to determine the toxicity of the synthesized QDs by in vivo imaging. Experiments were performed on water-soluble QDs (MPA-CdTe, MPA-CdTe/ZnS, MSA-CdTe, MSA-CdTe/ZnS, GSH-CdTe, GSH-CdTe/ZnS, TGA-CdTe, TGA-CdTe/ZnS, GSH-ZnSe and GSH-ZnSe/ZnS ) and toxicity was measured. Synthesized QDs were characterized by high intensity (fluorescence spectroscopy), FWHM and zeta potential (ZS Zetasizer) were selected due to their suitability for this task. The toxicity of QDs was determined by the MTT assay on the cell line HEK 293. The experiments show that a core/shell structure is less toxic than a core structure. The results indicate that the toxicity of our synthesized QDs is the lowest for MPA-CdTe (core structure) and MPA-CdTe/ZnS (core/shell structure).
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